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ixplore standard compound microscope system  (Olympus)


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    Structured Review

    Olympus ixplore standard compound microscope system
    Ixplore Standard Compound Microscope System, supplied by Olympus, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ixplore+standard+compound+microscope+system/IXplore+Standard+Compound+Microscope+System/pm41339605-52-8-13
    Average 95 stars, based on 26 article reviews
    ixplore standard compound microscope system - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: Afterward, the nuclei were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, #MG1789, MesGen Biotech, Shanghai, China) for 5 min, and the samples were mounted using anti-fade medium (#MFF1002, MesGen Biotech, Shanghai, China). .. Fluorescence imaging of phalloidin was performed using the IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). .. The number and relative size of phalloidin in each group were analyzed using ImageJ software.

    Article Title: NLRP3 blockade by MCC950 suppressed osteoclastogenesis via NF-κB/c-Fos/NFATc1 signal pathway and alleviated bone loss in diabetes mellitus.
    Article Snippet: Obesity and type 2 diabetes mellitus (T2DM) are linked to osteoporosis development, with obesity being a significant risk factor for T2DM.. T2DM patients with obesity exhibit a higher fracture rate and often have a poor prognosis post-fracture.. To address the urgent need for understanding the mechanisms of diabetic osteoporosis (DOP), research is ongoing to explore how obesity and T2DM impact bone metabolism.

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: .. Fluorescence imaging of ROS was performed using the IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). .. After being cultured in differentiation medium with or without LBT, cells underwent total RNA extraction using the TRIzol reagent (#CW0580, Cwbiotech, Beijing, China) according to the manufacturer’s protocol.

    Imaging:

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: Afterward, the nuclei were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, #MG1789, MesGen Biotech, Shanghai, China) for 5 min, and the samples were mounted using anti-fade medium (#MFF1002, MesGen Biotech, Shanghai, China). .. Fluorescence imaging of phalloidin was performed using the IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). .. The number and relative size of phalloidin in each group were analyzed using ImageJ software.

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: .. Fluorescence imaging of ROS was performed using the IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). .. After being cultured in differentiation medium with or without LBT, cells underwent total RNA extraction using the TRIzol reagent (#CW0580, Cwbiotech, Beijing, China) according to the manufacturer’s protocol.

    Microscopy:

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: Afterward, the nuclei were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, #MG1789, MesGen Biotech, Shanghai, China) for 5 min, and the samples were mounted using anti-fade medium (#MFF1002, MesGen Biotech, Shanghai, China). .. Fluorescence imaging of phalloidin was performed using the IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). .. The number and relative size of phalloidin in each group were analyzed using ImageJ software.

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: Subsequently, they were treated with a color-developing solution consisting of sodium nitrite (#A415326, Sangon Biotech, Shanghai, China) and pararosaniline chloride (#S19024, Yuanye Bio-Technology, Shanghai, China), incubating for 20 min in the dark. .. TRAP staining images were captured using the IXplore standard compound microscope system and the BX43 manual system microscope (Olympus Corporation, Tokyo, Japan). .. The area of TRAP-positive cells (with ≥ 3 nuclei) was quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: NLRP3 blockade by MCC950 suppressed osteoclastogenesis via NF-κB/c-Fos/NFATc1 signal pathway and alleviated bone loss in diabetes mellitus.
    Article Snippet: Obesity and type 2 diabetes mellitus (T2DM) are linked to osteoporosis development, with obesity being a significant risk factor for T2DM.. T2DM patients with obesity exhibit a higher fracture rate and often have a poor prognosis post-fracture.. To address the urgent need for understanding the mechanisms of diabetic osteoporosis (DOP), research is ongoing to explore how obesity and T2DM impact bone metabolism.

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: .. Fluorescence imaging of ROS was performed using the IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). .. After being cultured in differentiation medium with or without LBT, cells underwent total RNA extraction using the TRIzol reagent (#CW0580, Cwbiotech, Beijing, China) according to the manufacturer’s protocol.

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: .. Fluorescent images of p65 localization were captured using the IXplore Standard Compound Microscope System (Olympus Corporation, Tokyo, Japan). .. Quantitative data were obtained from a minimum of five biological replicates, analyzed using GraphPad PRISM 8 (GraphPad Software, San Diego, CA, USA), and displayed as bar graphs or dot plots representing the mean ± standard deviation (SD).

    Article Title: Transcriptome Analysis of Differentially Expressed Genes and Molecular Pathways Involved During Osteoclast Differentiation.
    Article Snippet: Bone remodeling relies on balanced osteoclast and osteoblast activity, with dysregulated osteoclast differentiation contributing to pathologies like osteoporosis.. While RANKL/M-CSF signaling and master regulators (NFATc1, c-Fos) are established, comprehensive temporal dynamics of gene networks governing progressive osteoclastogenesis stages remain poorly characterized.. We employed an integrated approach combining RANKL-induced osteoclast differentiation from murine bone marrow-derived macrophages (BMMs) with rigorous functional validation (TRAP staining, podosome visualization via rhodamine-phalloidin/DAPI, Western blotting for NFATc1/c-Fos/CTSK) and high-throughput RNA sequencing across critical time points (day 0, 1, 3, and 5).

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: .. Fluorescent images were captured with a IXplore standard compound microscope system (Olympus Corporation, Tokyo, Japan). ..

    Staining:

    Article Title: Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression
    Article Snippet: Subsequently, they were treated with a color-developing solution consisting of sodium nitrite (#A415326, Sangon Biotech, Shanghai, China) and pararosaniline chloride (#S19024, Yuanye Bio-Technology, Shanghai, China), incubating for 20 min in the dark. .. TRAP staining images were captured using the IXplore standard compound microscope system and the BX43 manual system microscope (Olympus Corporation, Tokyo, Japan). .. The area of TRAP-positive cells (with ≥ 3 nuclei) was quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Transcriptome Analysis of Differentially Expressed Genes and Molecular Pathways Involved During Osteoclast Differentiation.
    Article Snippet: Bone remodeling relies on balanced osteoclast and osteoblast activity, with dysregulated osteoclast differentiation contributing to pathologies like osteoporosis.. While RANKL/M-CSF signaling and master regulators (NFATc1, c-Fos) are established, comprehensive temporal dynamics of gene networks governing progressive osteoclastogenesis stages remain poorly characterized.. We employed an integrated approach combining RANKL-induced osteoclast differentiation from murine bone marrow-derived macrophages (BMMs) with rigorous functional validation (TRAP staining, podosome visualization via rhodamine-phalloidin/DAPI, Western blotting for NFATc1/c-Fos/CTSK) and high-throughput RNA sequencing across critical time points (day 0, 1, 3, and 5).



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